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Peer review Peer review information Nature Reviews Endocrinology thanks Mario Rotondi and the other, anonymous, reviewer(s) for their contribution to the peer review of this work

Takeda, K

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6) was purified by reversed-phase high-performance liquid chromatography (RP-HPLC) using a YMC Triart C8 column (10 250 mm, 5 m) (YMC, Kyoto, Japan) and loaded onto an activated Sep-Pak C18 (Sep-Pak light C18 cartridge: Waters Co., Milford, MA, USA)