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Mitochondrial mass by NAO staining ( i , n = 3 biological replicates) and mitochondrial imaging by TOM20 staining (representative images from n = 2) ( j ) in U2OS cells treated 5FU for 72 h, with mean s.e.m

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ALA targets neurotrophic factors such as nerve growth factor and metabotropic glutamate receptors through epigenetic mechanisms [72]

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Fresh isolation of white blood cells and ROS detection Blood samples were collected from rats before and 1 h after glucose challenge (gavage of 2 g/kg body weight)