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Free Radic Biol Med 49:14941504 Sedlak J, Lindsay RH (1968) Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellmans reagent

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In this method, the sample containing the antioxidant solution is mixed with the phosphomolybdenum reagent, which consists of 0.6 M sulfuric acid, 28 mM sodium phosphate, and 4 mM ammonium molybdate

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