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They also help maintain lipid metabolic crosstalk with mitochondria and the ER via membrane contact sites [189, 190]

The mitochondrial pellet was resuspended in MSHE buffer with BSA and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific)

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Patients who maintain adequate glutathione status during rapid weight loss report fewer subjective complaints and better preservation of lean mass, but the weight loss trajectory itself remains determined by caloric deficit and tirzepatide's appetite-suppressing mechanism