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In brief, for the HITI donor vector containing the sgAlb-E14 expression cassette and scExe4 donor fragment, the sgAlb-E14 expression cassette and scExe4 (NGF-FCS2-Exe4) sequence flanked by two sgAlb-E14 gRNA target sequences were prepared by PCR using PrimeSTAR GXL polymerase (Takara Bio)

Keeping the dynamic balance of cardiomyocytes plays a key role in maintaining the normal physiological function of heart (Ma et al., 2020), and the loss of cardiomyocytes caused by CD would induce and aggravate the development of HF

Its ability to promote wound healing and angiogenesis significantly exceeds that of standard angiogenic growth factors

Furthermore, these agents lead to a delay in gastric emptying [5], resulting in satiety and consequently reducing food intake

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