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In addition to H3K4me3, the transcriptional-repression mark histone H2AK119 mono-ubiquitination (H2AK119ub) is also involved in transcriptional regulation, always occurring together with H3K27me3 in the same chromatin regions ( Bdnf promoters I to IV, while BHBA treatment weakened the occupancy of H2AK119ub at these promoters (Figure 1D)