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First, GST weakens the efficacy of chemotherapy drugs by promoting their in vitro extrusion

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Additionally, HC treatment induced the upregulation of MTFR1 and MIEF1, proteins directly involved in mitochondrial fusion/fission, as well as in cell death, cancer cell proliferation, and metabolism (Wang et al., 2015

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In 3c-1 the cells are sedimented as a pellet and then resuspended in the solution of the required density, while in 3c-2 this pelleting step is omitted and the suspension mixed with a high-density solution