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Regulatory authorities discontinued early appetite-suppressant drugs, including fenfluramine and sibutramine, because of safety concerns (11)

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The blood samples were centrifuged (400 x g, 5 min, and 25 C), and the resulting red blood cell pellet was washed in RPMI-1640 medium (Sigma-Aldrich) and filtered through cellulose columns (Sigma-Aldrich) for leukocyte removal

The ability of these cells to migrate to tissues at risk of inflammation exacerbates local inflammatory responses, creating a cycle that can lead to further tissue damage and dysfunction