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In Brazil till July 2005, 139,678 donations were screened in pools of 6 to 12 donations by in-house RT-PCR method having sensitivity of 500 IU/ml for all six genotypes of HCV

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Collect the cell lysate and centrifuge at 13,200 g at 4 C for 15 min, and the supernatants were used for LCMS analysis after diluting with water to proper concentrations

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