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This mixture was then diluted in a 1:1 ratio with a dialysis buffer composed of 20 mM TRIS (pH 7.5), 200 mM NaCl, and 0.5 mM TCEP

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Recent studies have emphasized the dysregulation of the Treg/Th17 axis in Hashimotos thyroiditis, with a shift toward proinflammatory Th17 responses (e.g., IL-17, IL-22) and reduced regulatory T cell activity

50 In vitro experiments suggest that SEMA may participate in regulating the fibrosis process by improving factors such as inflammatory or metabolic triggers of fibrosis, like IL-6 and free fatty acids, as well as fibrotic structural features