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The membranes were blocked for 1 h at room temperature with 5% nonfat milk in tris buffered saline Tween-20 (TBST), then incubated overnight at 4 C with specific primary antibodies: anti-rabbit ALDH2 (1:1000, 15310-1-AP, Proteintech), anti-rabbit peroxiredoxin 6 (PRDX6) (1:1000, 13585-1-AP, Proteintech), anti-rabbit VDAC1 (1:1000, 55259-1-AP, Proteintech), anti-mouse glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:1000, 60004-1-lg, Proteintech), and anti-mouse tyrosine hydroxylase (TH) (1:1000, T2928, Sigma)
