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Note that semiquinone radicals can possess extremely long half-lives, up to days at 37 C 35 , and as such, it is unsurprising that SQ . is present over 30 minutes in the model (Fig

Iron measurement using the Iron Assay Kit For total iron measurement, the Iron Assay Kit (Sigma-Aldrich, MAK472) was used, according to the manufacturers instructions

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In vivo and in vitro models, it was found that in the IDH1 mutant glioma cells intervened by triptolide, the transcriptional activity of Nrf2 was suppressed, and the expression of genes related to glutathione anabolic metabolism (GCLC, GCLM, and SLC7A11) was significantly down-regulated