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Whole cell lysate proteins were extracted from the intestinal and muscle tissues, and cultured rat L6 myocyte cells using tissue-protein extraction reagent (T-PER) supplemented with proteinase and phosphatase inhibitors (all from Thermo Fisher Scientific, Inc.)

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Zhang, C., et al

Although FOXO4-DRI treatment did remove the senescent cells, the cartilage formation capacity of retained chondrocytes was not improved

Selective vulnerability and pruning of phasic motoneuron axons in motoneuron disease alleviated by CNTF