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64 Methylene tetrahydrofolate dehydrogenases (MTHFD1 in cytosol and MTHFD2 or MTHFD2L in mitochondria) catalyze the oxidation of 5,10-methylene-THF (CH2-THF) to form 10-formyl-THF, and 10-formyl-THF dehydrogenases (ALDH1L1 in cytosol and ALDH1L2 in mitochondria) catalyze the oxidization of 10-formyl-THF to generate CO 2 with concomitant NADPH production