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Paired-end FASTQ files were then run through the DADA2 pipeline (v1.8) to generate an amplicon sequence variant (ASV) table and assign taxonomy

How should it be taken

As we explained in the CY 2005 OPPS final rule (69 FR 65775), we generally use the lowest APC payment rate applicable for use with the nominated device when we assess whether a device meets the cost significance criterion, thus increasing the probability the device will pass the cost significance test

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However, our efforts often turn into frustration, and we may end up confused as to what to actually buy