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1a) is formed by interactions between the middle RHDs across the tetramer interface, with two PDO domains adopting a two-fold symmetric dimer at the top of each tetramer (see also Fig

In vitro and animal studies have found that lipoic acid exhibits immunomodulatory properties through mechanisms that stimulate the production of cyclic AMP (cAMP) (90, 91) a central regulator of innate immune functions and inhibits the migration of immune cells into the brain and spinal cord (92), possibly by decreasing endothelial expression of cell adhesion molecules, inhibiting expression of enzymes like matrix metalloproteinases (MMP), and/or reducing the permeability of the blood-brain barrier (87, 89, 93, 94)

ultra HPLC was coupled to an LTQ Orbitrap Elite (Thermo Fisher Scientific) MS