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Immunoprecipitation - Cecum, liver, and brain tissues were collected in IP lysis buffer (150 mM NaCl, 10 mM Tris-HCl, pH 7.4, 1% NP-40, 1 mM PMSF, and 1 mM protease inhibitor cocktail), homogenized, and incubated on ice for 20 min to ensure complete lysis

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Other infrequent side effects are nasopharyngitis, headache, infections in urinary tract, upper respiratory tract, and increased pancreatic enzyme (amylase and lipase) levels [4951, 59]