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Following cell lysis, the HaloTag fusion protein is covalently captured on the HaloLink Resin, and nonspecific proteins are washed away

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Therefore, the failure of the M2 shift is a synergistic outcome of both sustained pro-inflammatory drivers and an intrinsic inability to utilize the specific metabolic fuels necessary for the anti-inflammatory and pro-repair functions of M2 macrophages (Figure 3)

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