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The 16S data was tested for normality and alpha-diversity metrics were statistically compared using multiple unpaired t-tests, corrected for multiple comparisons using the HolmSidak method (GraphPad Software Inc., La Jolla, CA)
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For instance, GF mice have modified gut shape, characterized by diminished total intestinal mass, expanded cecum, shorter and thinner villi, and depleted mucus layers, decreased epithelial cell renewal, and impaired intestinal motility 12
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