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In the fifth aspect of the first embodiment of the present invention, the (3-31) amino acid fragment of Semaglutide is prepared by a recombinant process

The Bradford assay was used to determine protein concentration of cell extracts, from there samples with the same amount of protein were separated using SDS-PAGE technology and transferred onto a PVDF membrane

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doi: 10.1016/j.nut.2019.03.017 184 TanakaHGourleyDDDekhtyarMHaleyAP