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It is evident that the substitution of an amino function for the hydroxyl group on C2 and the methylation of N10 are the primary structural differences between MTX and the structure of naturally occurring folic acid (Rubino 2001)

2017;35(15_suppl):4087

10.1016/j.cej.2022.135723 [Google Scholar] Zhang M, Jiao P, Wang X, Sun Y, Liang G, Xie X, Zhang Y (2022) Evaluation of Growth performance, nitrogen balance and blood metabolites of mutton sheep fed an ammonia-treated aflatoxin B1-contaminated diet

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Oral administration has shown GI-local effects in rodent studies and some systemic effects