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Phosphorus also helps regulate pH balance and fluid homeostasis in the body
I.RamchunderZ.ChoiJ.ShchepinovaM
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The cells were blocked with 5% goat serum, 0.02% Triton X-100 in PBS for 30 min and then incubated for 12 h in phospho-Met (Tyr1234/1235) (D26) XP rabbit mAb diluted 1:1,000 in 1% goat serum in PBS at 4 C
Immunofluorescence The paraffin sections of colonic tissue were dewaxed and rehydrated in gradient alcohol, then the sections were deparaffinized with xylene and rehydrated, then submerged into EDTA antigenic retrieval buffer and microwaved for antigenic retrieval
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