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Additionally, the third amino acid in the native human or sheep sequence, glutamic acid, is replaced with an arginine in LR3 IGF-1, thus substituting a negatively charged amino acid in place of a positively charged amino acid

The samples were diluted to concentrations between 600 and 1400 g/mL in 50% methanol

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Their job is to transport IGF-1 through the circulation while keeping it in an inactive, receptor-inaccessible form