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To isolate chondrocytes, fresh articular cartilage tissues were rinsed with basal medium (BM), consisted of high glucose Dulbeccos modified Eagles medium (DMEM, Gibco/Thermo Fisher Scientific, Waltham, MA, United States), with 2 Antibiotics-Antimycotics (anti-anti, Life Technologies, Carlsbad, CA, United States) and then cut into 1 mm 3 pieces

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This results in them eating less leading to quick and often quite dramatic weight loss

These fractures typically occur in the subtrochanteric or femoral shaft region and may be heralded by prodromal thigh or groin pain