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2.4 Bioluminescence resonance energy transfer assays To generate dose-response curves, HEK-293 cells were transiently transfected with equal amounts of labeled receptors and labeled G protein for G protein recruitment assay and in a 1:2 ratio for arrestin and KRAS assays

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The body may release large amounts of a hormone called corticotropin-releasing factor (CRF) from the hypothalamus, stimulating the adrenal cortex