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Images were captured using a camera (Evolve 512 Delta, Photometrics), acquisitions were saved and data were logged using Metamorph software (Molecular Devices)

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However, in group 4 (long-term diabetic group), there was a significant difference between the two patterns in insulin-positive islets ( p = 0.04) and not in insulin-negative islets ( p = 0.06)

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