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Kokot A, Zlatar M, Stupnisek M, et al

To do this, they created a sort of evil FOXO4 twin called a FOXO4-DRI peptide it looked and acted like FOXO4 from a chemical perspective (in fact, p53 liked to bind with FOXO4-DRI even more than regular FOXO4, which is important because of some complicated chemistry), but FOXO4-DRI didnt only live in the nucleus it could exist anywhere in the cell

No definitive research establishes optimal timing, leaving this decision to individual preference and circumstance