Chemical treatments Yeast strains were grown to midlog phase, 21 OD 600 units of cells were harvested and centrifuged at 4700 g for 2 min at 25C
Estradiol (E2) activates the enzymes GCL and GPX, as well as activating CBS directly
Low-temperature storage conditions further help preserve peptide integrity during long-term storage
Frozen BAC water must be discarded, not thawed and used
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