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Melatonin (N-acetyl-5-methoxytryptamine) is an endogenously produced indoleamine that can modulate many important physiological reactions in the body 11

2.7 Aortic ring assay The mouse aortic ring assay was conducted according to a previously described method ( 2.8 Western blotting Proteins were extracted from the tissue samples, C2C12 myocytes, or HUVECs using RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Nacalai Tesque, Kyoto, Japan), separated using SDS-PAGE, and transferred to PVDF membranes

10.3389/fphys.2021.669356 Front Physiol

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