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The plate containing the frozen cells were lyzed with Pierce RIPA Lysis and Extraction Buffer (Thermo Fischer Scientific) supplemented with 1 cOmplete Protease Inhibitor Cocktail (Roche) (220 L/well) for 10 min on ice on a tilting board

To discuss your treatment options-whether you are comparing semaglutide vs lifestyle interventions , exploring the pros and cons of medication, or considering your next steps in the GLP1 weight loss comparison -we are here to help

Your prescriber will assess your medical history and other treatments to decide whether this treatment is suitable for you