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Cells were lysed for 15 minutes in ice-cold RIPA buffer consisting of 1% NP40, 0.1% SDS, 0.5% deoxycholate in PBS, supplemented with 1 mM PMSF, 1X cOmplete, EDTA-free Mini Protease Inhibitor Cocktail (Sigma Cat#11873580001) and 1X PhosSTOP (Sigma Cat#4906845001)

Actives that disrupt the barrier, cause irritation, or cannot be used consistently tend to fail, not because they dont work, but because skin cant sustain them

However, a healthy liver and lifestyle choices remain essential for optimal detoxification

Pathway analysis indicated that 47 of the 94 genes are involved in metabolic pathways such as ferroptosis, D-glutamine and D-glutamate metabolism, and autophagy (Figure 3C)