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Laparoscopic cholecystectomy in the treatment of acute cholecystitis: comparison of results between early and late cholecystectomy

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After three washes with PBS, the sections were pre-incubated in blocking solution (5% serum albumin, 2% normal goat serum, and 0.3% Triton X-100 in PBS) for 20 min at room temperature, then incubated with an anti-c-Fos rabbit antibody (#sc-52, 1:2000

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Spinach and kale contain vitamins A and E, aiding in skin repair and elasticity maintenance