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The prescribing clinician should review the reason for treatment, side effects, diabetes medicines, weight trajectory, pregnancy plans, procedures, and long-term maintenance

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After each run was completed, the column was re-equilibrated with 1% B before the next injection

Secondary antibodies were AlexaFluor-488 goat anti-mouse (Invitrogen A11001, lot 2513496), AlexaFluor-647 goat anti-rabbit (Invitrogen A32733, lot XG349344), AlexaFluor-488 goat anti-rabbit (Invitrogen A11008, lot 2420731) all diluted 1:500

The mice were allowed to move freely between the chambers, and their positions were recorded using an overhead camera