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Targeted analysis was performed using the same chromatographic conditions but with the instrument operating in a parallel reaction monitoring (PRM) method in which targeted HCD MS2 scan were acquired on 12 precursor ions, corresponding to 3 proteins, using an isolation windows of 0.7 m/z (Supplementary Data 1)

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This parent-protein halo is exactly parallel to the full-length hormone halo that inflates claims for other peptide fragments: the reputation of the large, well-studied molecule is borrowed to dress up a smaller, less-studied stand-in