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ECs were treated with IL-1 (100 ng/ml) for 24 h with or without 50 M ferroptosis inhibitor ferrostatin-1 or 10 M ferroptosis activator erastin at 37C, rinsed with PBS, fixed with 4% paraformaldehyde for 10 min and permeabilized with 0.1% Triton X-100 (Beyotime Institute of Biotechnology) for 5 min at 25C

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Injury severity and chronicity do

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-Glutamyl transpeptidase (GCLC) deficiency has been found in patients with CNS involvement and glutathionuria