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Its sequence is alanine-histidine-lysine bound to a copper(II) ion

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Pulsatility is thought to matter for the downstream physiological effects of GH, which is part of why research models favor a short GHRH signal (no-DAC) that reinforces natural pulses over a multi-day DAC signal that raises the baseline more persistently

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After washing in PBS, horseradish peroxidase-conjugated goat anti-rabbit IgG (ab175470, Abcam) diluted 1:200 in 1% rabbit serum in PBS was applied, and the slides were incubated for 30 min at room temperature