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Plasmid transfection and luciferase reporter assay The luciferase-based reporter plasmids pNFKB-Luc and pTA-Luc (Takara Bio, San Jose, USA) were applied with Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturers instructions

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Unlike full HGH, it does not raise IGF-1 levels significantly , so its unlikely to have the side effects associated with growth hormone therapy (like blood sugar swings or joint pain)

Practitioners may administer them once or twice weekly