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Furthermore, both GIP 100 nM and Tirzepatide at 10 nM stimulated lipolysis through cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) pathway, as evidenced by increased glycerol release and HSL phosphorylation on day 19 differentiated cells

Group 3 samples were maintained in cryotubes and stored in liquid nitrogen for further RNA and protein extraction

Back O'Rahilly, S (15 April 2020' 'The islet's bridesmaid becomes the bride: Proglucagon-derived peptides deliver transformative therapies', Cell , 184, 1945-48