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GR activity assays GR can catalyze the reaction as follows: NADPH + H + + GSSG NADP + + 2GSH, and the oxidation of NADPH can be measured by the decrease in absorbance at 340 nm ( = 6.22 10 3 M 1 cm 1 )

Think of the IRR as a "Master Switch" for healing that exists on the surface of cells, particularly immune cells and nerve cells

The resulting cells were fixed in 4% paraformaldehyde and incubated with a blocking buffer for 1 h, and the cells were permeabilized with 0.2% Triton and 0.1% Tween 20 in a blocking buffer (3% bovine serum albumin in PBS) for 2 h