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Mice were acclimatized to the animal facility for 1 week before they received injections of MCF-7:2A human breast cancer cells: 2 10 7 cells were resuspended in 100 L PBS (Collaborative Biomedical Products, Bedford, MA, USA) and were bilaterally injected into the mammary fat pads of 20 ovariectomized mice

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Zhu X, Kalyanaraman N, Subramanian R

If you notice a small amount of powder on the rubber stopper, you can gently invert the vial a few times to allow the solution to wash over and dissolve it