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Direct-zol RNA MiniPrep kit was purchased from QIAGEN and nCounter GX Human Immunology v2 panel from nanoString Technologies

BPC-157 is soluble in DMSO at concentrations up to approximately 5 mg/mL, and DMSO is typically selected when preparing concentrated stock solutions for cell culture assays where final DMSO concentration in the well can be kept below 0.1% to avoid cytotoxicity artifacts

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Within the circulation, more than 99% of IGFs are bound to specific high-affinity IGF-binding proteins (IGFBPs), six of which have been identified, regulating their half-life and bioavailability [7]