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Quantitative real-time PCR (qPCR) for assaying relative expression of TMV-CP and tobacco defense genes ( NtPR1-a , NtPRB-1b , NtGSTtau , and NtGSTphi ) was conducted with the 2SYBR FAST Readymix reagent (KAPA Biosystems, USA)

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Patients might lack vitamins and minerals like vitamin B12, iron, calcium, and vitamin D