We chose vitamin E deficiency, as this was understood to be a significant contributor to the pathogenesis of BBS, and celiac disease status, as this was indicated to be the primary diagnosis in several BBS cases [51], based on our literature search
Results & discussion Method validation The HPLC method was validated as to specificity, linearity, sensitivity, accuracy, precision (repeatability and reproducibility), LOD, LOQ, and stability as per the ICH [50]ICH Q2 (R1), Validation of analytical procedures: text and methodology, in Proceedings of the International Conference on Harmonization, Geneva, Switzerland, 2005
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Twelve (50%) patients in the proactive group and nine (36%) patients in the reactive group were at high risk of ODS