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J Appl Physiol 117(3):223230 Xia P, Chen J, Liu Y, Fletcher M, Jensen BC, Cheng Z (2020) Doxorubicin induces cardiomyocyte apoptosis and atrophy through cyclin-dependent kinase 2mediated activation of forkhead box O1

For fluorescence microscopy studies, HL-60 cells and HepG2 cells (seeding densities 1 10 4 cells/well and 8 10 4 cells/well, respectively) were placed in serum-free medium to facilitate their adhesion to coverslips and cultured on lysine-coated 8-well chamber slides (IBIDI) following the procedure detailed by 2.8 Cell surface binding, endocytosis, and downregulation of TfRs For surface binding and endocytosis of AF-labeled proteins, the cells were rinsed twice with ice-cold HEPES-buffered, serum-free media, pH 7.4, and incubated for 1 h on ice with either 500 nM hemeAF546HPX, hemeAF546HPX, or AF488 holo-human Tf

This difference was not statistically significant, most probably due to sample size

Deficiency treatment is essential because it helps to alleviate fatigue and neurological disorders

Available in both subcutaneous and oral formulations