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Protein Gel Analysis The crude extracts from the TMABA dehydrogenase activity assay were further analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) as described elsewhere (Green et al., 2012) using the Mini-PROTEAN Tetra Cell #1658000EDU System from Bio-Rad Laboratories Inc

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The expression levels of IL-6, IKK- and NF-B were elevated in pancreatic cells from T2DM rats, accompanied by increased Bax expression and decreased Bcl-2 expression, as well as decreased pancreatic -cell function