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109 , with the modification that no Matrigel was added after day 40 of differentiation to medium IDM + A (1:1 of DMEM/F12 and Neurobasal, 0.5% N2 supplement, 2% B27 +vitamin A, 0.25% insulin solution, 50 M 2-mercaptoethanol, 1% Glutamax, 0.5% MEM-NEAA, 1% penicillin-streptomycin, 0.4 mM vitamin C, and 1.49 g HEPES per 500 ml)

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901 Osteoclasts are giant, multinucleated cells that arise from the fusion of monocytes/macrophage progenitor cells differentiated from myeloid progenitors in bone marrow