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Rendn-Ramirez A, Cerbn-Solrzano J, Maldonado-Vega M, Quintanar-Escorza MA, Caldern-Salinas JV

Membranes were blocked in 5% (w/v) milk in PBS-T (PBS with 0.1% [v/v] Tween-20) for 1 h, incubated overnight at 4 C with the primary antibody in the same blocking solution, washed three times with PBS-T for 5 min, incubated with HRP- or Alexa Fluor (AF)-conjugated secondary antibody (where indicated) in 0.1% (w/v) milk in PBS-T for 1 h and, washed three times with PBS-T for 5 min

When someone arrives with a significant deficiency, their stores are low, their red blood cells may be enlarged (macrocytosis), and, in longstanding cases, nerve function can be affected

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