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Then, 50 l of enzyme dilutions were incubated for 10 min at each temperature point into nonskirted 96-well PCR plates (VWR, 732-2387) sealed with thermoresistant adhesive sheets (Thermo Scientific, AB-0558)
Large bubbles or persistent foam suggest the reconstitution was too aggressive
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REPUTATION FOR INTEGRITY Ukraines army remains plagued by regular reports of abuse, corruption and mismanagement in both recruitment and throughout the ranks
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