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synthetic analogs may be used in conjunction with mineralocorticoids where applicable

Fat loss in certain areas can alter the proportions and shape of the face, the size of the lips, cheeks and chin, altering the characteristics and thus, resulting in facial aging [5,6,7]

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The oocytes were subsequently incubated in a permeabilizing solution (0.5% Triton-X-100 in PBS-PVA) for 30 min at room temperature, washed twice in PBS-PVA, and then blocked with 2% FCS in PBS for 45 min