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Total RNA was extracted using the Maxwell RSC Simply RNA Tissue Kit (Promega

The key distinction is that semaglutide does not affect thyroid hormone production or functionit addresses weight through a separate biological pathway

Two investigators independently assessed study quality, compared results, and resolved any inconsistencies through discussion until reaching a consensus

Analysis of clinical chemistry parameters measured at the end of the 4-week study (Supplementary Table 11) showed that MEDI4166 treatment reduced FPG, total cholesterol and serum alanine transaminase (ALT) levels, which is indicative of improved liver function